SELECTIVE EXTRACTION OF ISOLATED MITOTIC APPARATUS
نویسندگان
چکیده
منابع مشابه
Selective Extraction of Isolated Mitotic Apparatus
Mitotic apparatus isolated from sea urchin eggs has been treated with meralluride sodium under conditions otherwise resembling those of its isolation. The treatment causes a selective morphological disappearance of microtubules while extracting a major protein fraction, probably consisting of two closely related proteins, which constitutes about 10% of mitotic apparatus protein. Extraction of o...
متن کاملSELECTIVE EXTRACTION OF ISOLATED MITOTIC APPARATUS Evidence That Typical Microtubule Protein Is Extracted by Organic Mercurial
Mitotic apparatus isolated from sea urchin eggs has been treated with meralluride sodium under conditions otherwise resembling those of its isolation. The treatment causes a selective morphological disappearance of microtubules while extracting a major protein fraction, probably consisting of two closely related proteins, which constitutes about 100/0 of mitotic apparatus protein . Extraction o...
متن کاملThe structure and some properties of the isolated mitotic apparatus.
The morphology of the isolated sea-urchin mitotic apparatus (MA) was examined by light and electron microscopy. With the polarization microscope and the Nomarski differential interference microscope, the isolated MAs appeared to be similar to in vivo MAs. Electron microscopy of the isolated MAs revealed the presence of microtubules, ribosome-like particles and vesicles. A close association betw...
متن کاملSpindle birefringence of isolated mitotic apparatus analysed by pressure treatment.
Sea-urchin zygote mitotic apparatus (MA) isolated in a glycerol/dimethylsulphoxide medium were treated with pressure. Pressure treatment had no effect on spindle birefringence when MA were in full-strength isolation medium. After placing MA in quarter-strength isolation medium, pressures of 4-0 X 10(3)-1-8 X 10(4) lbf in.-2 (2 X 76 X 10(4)-I X 24 X 10(5) k N m-2) for 15 min caused reduction of ...
متن کاملImmunochemical Studies of 22s Protein from Isolated Mitotic Apparatus
Evidence is presented that the "22S protein" of mitotic apparatus isolated from sea urchin eggs is not microtubule protein. An antibody preparation active against 22S protein is described, and immunochemical studies of the distribution of 22S protein in various cellular fractions and among morphological features of mitotic apparatus are reported. The protein is ubiquitous in the metaphase egg f...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1971
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.48.2.324